金月芽期刊網

和“pepo”相關的論文

  • 西葫蘆未受精胚珠離體培養條件的優化及胚囊植株的產生 相關:西葫蘆(Cucurbita pepo L.)
  • 【目的】建立西葫蘆離體雌核發育高頻植株再生體系,以加速自交系選育,有效縮短西葫蘆雜種一代育種周期。【方法】將西葫蘆未受精胚珠接種至附加2,4-D、NAA、BA的N6培養基上可形成胚狀體,該胚狀體轉接至無激素的N6培養基上可形成再生植株。【結果】試驗共獲得120棵再生植株(R0),其中41株因生活力低下死亡,8株用于摸索移栽方法死亡,71株移栽成活且生長正常,其中42株性狀表現符合二倍體特征且育性正常,已有10株獲得自交果實及種子,其余29株育性異常。試驗結果表明,胚珠發育時期、培養基、供體基因型及供體栽培季節等均顯著影響胚狀體誘導頻率。【結論】胚狀體起源于胚囊成員細胞,再生植株為胚囊植株。試驗篩選出3種誘導頻率較高的培養基;以開花前1日及當日的胚珠誘導頻率較高;秋播材料誘導效果最好。
  • 溫度和濕度對西葫蘆貯藏過程中品質的影響 相關:西葫蘆(Cucurbita pepo L.)
  • 研究了室溫(28-32℃)和10℃條件下不同濕度(低濕、飽和濕度)對西葫蘆(Cucurbita pepo L.)采后貯藏品質的影響。結果表明,隨著貯藏時間的延長,西葫蘆表皮顏色趨于黃化,水分含量下降,萎蔫和腐爛程度加深。低溫和飽和濕度處理可以延緩顏色指數、萎蔫指數和腐爛指數的上升以及葉綠素、維生素C和可溶性蛋白含量的下降,減少西葫蘆瓜肉和表皮水分的損失,較好地保存西葫蘆的外觀和營養品質。
  • 蚯蚓糞在西葫蘆穴盤育苗中的應用 相關:蚯蚓糞 西葫蘆(Cucurbita pepo
  • 以蚯蚓糞為主要原料配合不同體積比的珍珠巖作為育苗基質,研究了復合基質對西葫蘆(Cuc urb ita pepo L.)種子萌發及幼苗生長發育的影響。結果表明,與草炭相比,蚯蚓糞具有有機質含量高,營養豐富,氮、磷、鉀含量高等特性;蚯蚓糞和珍珠巖以3:1(V/V )混合基質育苗效果最佳,既提高了西葫蘆種子的出苗率,又促進了幼苗的生長發育,地上部干重、地下部干重、根系活力和壯苗指數分別是對照的1.95、2.20、1.34和2.20倍。
  • 西葫蘆穴盤育苗潮汐灌溉技術研究 相關:西葫蘆(Cucurbita pepo L.)
  • 以西葫蘆(Cucurbita pepo L.)為試材,采用潮汐灌溉和頂部灑水(CK)2種灌溉方式,其中潮汐灌溉有2個灌溉時長水平:15 min/次、30 min/次,3個灌溉頻率水平:1次/d、1次/2 d、1次/3 d,共7個處理;探討了潮汐灌溉和頂部灑水2種灌溉方式對西葫蘆穴盤苗生長及生理變化、基質電導率、含水量和灌水量的影響。結果表明,與頂部灑水灌溉方式相比,潮汐灌溉的15 min/次、1次/d處理和30 min/次、1次/d處理的穴盤苗素質最佳,且分別節水10%、6%;最優潮汐灌溉制度是灌溉高度為穴盤高度的2/3(3 cm)、灌溉時長為15 min/次、灌溉頻率為1次/d。
  • 痕量灌溉技術在日光溫室西葫蘆栽培上的應用研究 相關:痕量灌溉 西葫蘆(Cucurbita pepo
  • 以西葫蘆(Cucurbita pepo L.)為材料,微噴灌溉為對照,研究痕量灌溉管不同埋深對日光溫室春季西葫蘆生長、產量和水分生產效率的影響,結果表明,痕量灌溉管不同埋深處理后對西葫蘆的生長影響無差異性;與對照相比,埋深6、12 cm 2個處理的西葫蘆葉片蒸騰速率分別降低了1.26、1.72 mmol(H2O)/(m^2·s),葉片凈光合速率分別提高了1.40、1.09μmol(CO2)/(m^2·s),果實有機酸含量分別降低了17.2%、6.9%;微噴灌溉的果實VC含量分別比2個處理降低了32.7%、30.5%。在0-6 cm深基質里含水量高低表現為微噴灌溉〉痕量灌溉管埋深6 cm〉痕量灌溉管埋深12 cm,在6-12 cm深基質里含水量高低表現為微噴灌溉〉痕量灌溉管埋深12 cm〉痕量灌溉管埋深6 cm;痕量灌溉管埋深6、12 cm的處理其667 m^2的耗水量分別比微噴灌溉下降了65.7%、61.7%,水分生產效率分別增加了102.4%、88.1%。綜合產量、耗水量等經濟因素,西葫蘆基質栽培的痕量管埋深以6 cm為宜。
  • 西葫蘆莖尖離體培養和快速繁殖技術 相關:西葫蘆(Cucurbita pepo L.)
  • 以西葫蘆莖尖為外植體,MS為基本培養基,探索了不同消毒處理、不同濃度的植物生長調節劑及其組合對西葫蘆莖尖再生和快速繁殖的影響。結果表明:西葫蘆莖尖在含有0.02%升汞與10%次氯酸鈉的溶液中消毒10min的效果最好,污染率降低到6.7%,成活率可達91.7%;西葫蘆莖尖誘導再生芽的最好培養基處理為MS+6-BA 0.5mg/L+IBA 0.10mg/L;最佳的生根誘導培養基為MS+IBA 0.30mg/L。
  • PepO通過上調腹腔巨噬細胞中MircroRNA-155的表達來抑制IL-6的產生 相關:PepO MircroRNA-155 MYD88
  • 探索microRNA-155在細胞炎癥反應調控中的作用機制。采用重組PepO蛋白刺激小鼠腹腔巨噬細胞(PEMs)誘導的microRNA-155(miR-155)及其對IL-6的調節。PepO刺激腹腔巨噬細胞誘導miRNA-155呈劑量和時間依賴性表達;Mimic和inhibitor轉染實驗顯示過表達mi R-155可抑制IL-6及My D88的表達;IL-6及MyD88與PepO也呈劑量依賴性;且IL-6與MyD88存在PepO處理時間的相關性。這些結果提示,PepO刺激腹腔巨噬細胞誘導miRNA-155可能通過靶向myD88通路信號從而抑制IL-6的表達,避免宿主不至于過度炎癥反應導致炎癥損傷。
  • Fatty Acid Accumulation in Seeds of Three Seed Pumpkin Cultivars 相關:SEED PUMPKIN CUCURBITA
  • In the literature there are many reports on the composition and properties of pumpkin seed oil; however, few is known about the effect of different stages of seed development on various fatty acid profiles in developing seeds. The objective of this study was to provide the changes of various fatty acid accumulations in seed oil obtained from the seeds of three pumpkin varieties belonging to the species Cucurbita maxima and Cucurbita pepo. Unsaturated acids (oleic and linoleic) were dominant in various fatty acids, which constituted 38.9%-49.1% and 29.4%-42.7% of the total fatty acids at seed maturity for three pumpkin varieties, respectively, while other fatty acid concentrations except for palmitic acid all did not reach 10%. Different varieties exhibited greater effect on various fatty acid contents and the total fatty acid contents in the seeds of pumpkin rather than the species. On the whole, palmitic acid profiles of the seed oil in three varieties all followed the fluctuant decrease during all the stages of seed development, but palmitoleic acid and the total fatty acid profiles of the seed oil in three varieties were just the opposite. Stearic, oleic and linoleic acid profiles of the seed oil in three varieties all experienced the third pattern that fluctuated during all the stages of seed development, but no significant differences in these three fatty acid concentrations were found from the beginning to the end. Linolenic acid concentrations of three varieties were on the decline and ultimately close to zero. Myristic and arachidic acid profiles of the seed oil followed different trends in three varieties. Among them, myristic and arachidic acid profiles of the seed oil of Yinhui-1 fluctuated downward all the time until seed maturity, but those of 0238-1 and Jinhui-2 completely changed oppositely.